Intercellular uptake of technetium-99m pertechnetate by different types of cell lines
Abstract
ABSTRACT
The purpose of this study is to determine the technetium-99m pertechnetate (99mTcO4) intercellular uptake by different types of cell lines. HeLa, human fetal osteoblast (hFOB), glial and glioma cell lines grown in 6-wells culture plates were incubated with 99mTcO4 of activity of 200, 400, 600, 800 and 1000 µCi for 30 minutes at 37oC and 5% CO2 humidified atmosphere. After incubation, the cells were washed 3 times with phosphate buffer saline to remove the extracellular traces of 99mTcO4. Measurements of the intercellular 99mTcO4 radioactivity were performed using single head gamma camera and the percentage uptake of the 99mTcO4 into the cells was calculated. The intercellular uptake of 99mTcO4 was found to be inversely correlate to the radioactivity. HeLa cell shows the highest uptake followed by hFOB, glial and glioma cell lines. Comparison of uptake between normal and cancer cells present indistinguishable results. The findings of this study suggest that the intercellular uptake of 99mTcO4 is highly dependent on the type of cells despite no significant different of uptake was found between normal and cancer cell lines. The level of radioactivity is also an important determinant factor that influence the uptake of 99mTcO4 into the cells. This study will be the first precedent toward understanding the cellular characteristic and pharmacokinetic of non-invasive imaging tracer for future molecular imaging and therapy.
ABSTRAK
Kajian ini bertujuan untuk mengenal pasti kadar penyerapan intersel technetium-99m pertechnetate (99mTcO4) oleh jenis sel yang berbeza. Kumpulan sel HeLa, sel human fetal osteoblast (hFOB), sel glial dan sel glioma dikultur dalam piring kultur dan diinkubasi bersama 200, 400, 600, 800 and 1000 µCi 99mTcO4 selama 30 minit (37oC, kelembapan atmosfera CO2 5%). Selepas proses inkubasi, sel dibasuh dengan phosphate buffer saline untuk membuang sisa-sisa ekstrasel 99mTcO4. Pengukuran radioaktiviti 99mTcO4 intersel dilakukan menggunakan kamera gamma, kemudian peratusan serapan 99mTcO4 oleh sel-sel dikira. Hasil kajian menunjukkan kadar serapan intersel 99mTcO4 berkadar songsang dengan radioaktiviti. Sel HeLa menunjukkan kadar serapan yang lebih tinggi berbanding sel hFOB, diikuti dengan sel glial dan sel glioma. Didapati tiada perbezaan kadar serapan antara sel kanser dan sel sihat. Konklusinya kajian ini menunjukkan bahawa kemungkinan kadar serapan intersel terhadap 99mTcO4 sangat bergantung kepada jenis sel, namun tiada perbezaan signifikan ditunjukkan apabila sel sihat dan sel kanser dibandingkan. Paras radioaktif juga merupakan factor yang penting dalam mempengaruhi serapan 99mTcO4 oleh sel.
