Diabettertm reduces post meal hyperglycemia via enhancement of glucose uptake into adipocytes and muscles cells

Abstract

ABSTRACT

There are lots of herbal products for diabetes mellitus treatment available in local market. Most of these products are not standardized and lack of efficacy and safety data. DiaBetter^TM is one of the herbal products that have been used for diabetes treatment. This study was carried out to determine the efficacy of DiaBetter^TM in reducing hyperglycemia and to elucidate the mechanisms by which hyperglycemia is reduced. The results showed that DiaBetter^TM significantly reduced post meal hyperglycemia in normal and diabetic rats, and improved glucose tolerance activity in diabetic rats particularly after 4 and 6 hours of administration. Antihyperglycemic mechanisms elucidation revealed that the DiaBetter^TM significantly enhanced insulin-stimulated glucose uptake into adipocytes and muscle cells, with the highest magnitude of enhancement were 1.54-fold (p<0.01) and 1.46-fold (p<0.001), respectively. Molecular mechanisms that responsible for this enhancement were the increment of insulin sensitivity at cells membrane. Cytotoxic evaluation was also done and confirmed that DiaBetter^TM was toxicologically safe against muscle and adipocytes cells. In conclusion, post-meal antihyperglycemic and glucose tolerance activity of DiaBetter^TM was mediated through the enhancement of glucose uptake into adipocytes and muscle cells. Insulin sensitizing activity showed by DiaBetter^TM suggests that this product has the potential to ameliorate insulin resistance condition. Therefore, it is suggested that DiaBetter^TM can be used as dietary adjunct for the management of type 2 diabetes mellitus which related to insulin resistance.

ABSTRAK

Terdapat banyak produk herba untuk rawatan diabetes melitus didapati di pasaran tempatan. Kebanyakan daripada produk-produk ini tidak diseragamkan dan kekurangan kemujaraban dan data keselamatan. DiaBetterTM ialah salah satu produk herba yang telah digunakan untuk rawatan penyakit kencing manis. Kajian ini dijalankan untuk menentukan kemujaraban DiaBetterTM dalam mengurangkan hiperglisemia dan menjelaskan mekanisme yang mana hiperglisemia dikurangkan. Keputusan menunjukkan bahawa DiaBetterTM dikurangkan dengan nyata hiperglisemia hidangan jawatan di tikus-tikus diabetes dan normal , dan meningkat kegiatan toleransi glukosa di tikus-tikus diabetes terutamanya selepas 4 6 jam pentadbiran. Mekanisme-mekanisme Antihyperglycemic penjelasan mendedahkan bahawa DiaBetterTM nyata sekali ditingkatkan pengambilan glukosa dirangsang insulin ke dalam adipocytes dan sel otot, dengan magnitud tertinggi peningkatan 1.54 (p<0.01 ) kali ganda dan 1.46 lipatan, masing-masing. Mekanisme molekul yang bertanggungjawab dalam peningkatan ini ialah tambahan kepekaan insulin di membran sel. Penilaian sitotoksik juga dibuat dan mengesahkan bahawa DiaBetterTM ialah toxicologically selamat menentang otot dan sel-sel adipocytes. Dalam kesimpulan, pasca hidangan antihyperglycemic dan kegiatan toleransi glukosa DiaBetterTM didamaikan melalui peningkatan pengambilan glukosa ke dalam adipocytes dan sel otot. Insulin memekakan kegiatan ditunjukkan oleh DiaBetterTM menandakan produk ini mempunyai potensi memperbaiki keadaan ketahanan insulin. Lantarannya, ia dicadangkan supaya DiaBetterTM boleh digunakan sebagai tambahan pemakanan untuk pengurusan 2 diabetes melitus, jenis yang mana berkaitan dengan ketahanan insulin.